Previous Works
Manage projects | Handle collaborations | Supervise PhD students
Experimental Design | Data Analysis | Presentation & Publication

PhD Thesis

GIGA-Research, Unit of Human Genetics (Prof V.Bours)

October 2006 – November 2014

Cancer characterization

The intra-tumor heterogeneity bias

Relapses are inevitable for many types of tumors. Such events are thought to be the consequence of cell sub-populations that are resistant to therapy. My project aimed at describing the bias coming from the internal heterogeneity of tumors for the early identification of treatment-resistant sub-populations, and thus improves the design of future therapies.

Computational & Molecular Biologist

  • Production of High-Throughput (HT) data from DNA & RNA
  • Management and Storage of HT data
  • Genome and Network Analyses of HT data
  • Statistical analyses
  • Coding using R and bioconductor, Python & Perl
  • Production of meaningful graphs
  • DNA / RNA Preparation (Extraction & QC)
  • FISH, Western Blot

PhD Collaboration

MRC, Imperial College London, C3-Neuroscience Lab (Prof F. Turkheimer)

September 2009 – December 2009

Wavelet smoothing

Statistical analyses of microarray signals

The aim of this project was to adapt the wavelet denoising methods to enhance the analysis of CGH microarrays. The resulting signal was then used to analyze the clonal heterogeneity of tumor samples.

Computational Statistics

  • Simulations of heterogeneous signal mixtures.
  • Assessment of tumor sub-population proportions.
  • Analysis using Gaussian mixture models using Expectation Maximization.
  • Smoothing using CHROMOWAVE Wavelet smoothing algorithm
  • Coding using MATLAB, R and bioconductor

Master Thesis

ULG, Mass Spectrometry Laboratory (Prof E. DePauw).

January 2006 – September 2006

Single Cell Fingerprinting

Using MALDI-TOF

The aim of this thesis was to identify the pattern of proteins expressed from distinct intact single tumor cells. Using MALDI-TOF, tumor cells are analyzed after cell-culture. This is quick and powerful method to identify the class and the grade of each tumor sample.

BioChemist

  • In vitro expansion of immortalized cell-lines (MCF7, MDA, LNCAP)
  • Spheroid culture of LNCAP cells
  • Protein extraction
  • Engines: MALDI-TOF & Nano HPLC coupled with Easy-Q-TOF
  • Immuno-Staining, Western Blot & 2D Gels assays

Master Internship

CNRS, Laboratoire de NeuroImmunologie des Annélides (Prof M.Salzet)

October 2004 – August 2005

Role of gralunin in Hirudo Médicinalis.

Cloning and Expression of a gralunin of Hirudo Médicinalis.

My aim in this project was to clone the leech GRN gene into a E. coli plasmid and to validate its insertion using sanger sequencing. Due to its action on the cell-cycle, this granulin was then over-expressed into neurons to study neural regeneration. This study led to a better understanding of the underlying mechanisms triggered by the granulin and thus a better understanding of the main biological pathways.

Molecular Biologist

  • Sanger Sequencing
  • DNA extraction
  • PCR
  • Cell culture
  • Clonal Selection

Antibody Adviser

Antibody-Adviser.org NPO

December 2012 – Present

Antibody Advisor NPO

Find, rate and manage antibodies

Antibody-Adviser.org is the faster social way to find and share reviews about antibodies. It offers an independent online resource of commercially available antibodies that have been tested by scientists and gives the opportunity to share personal experiences with the scientific community.

Co-funder & Web developer

My Skills

Select the field.

NGS 80%
Microarrays 100%
Statistics 70%
Advanced Stats 70%
Advanced Stats 70%

Nothing in the biology makes sense except in the light of evolution.

sub specie evolutionis

- Theodosius Dobzhansky (1973) -

Get in touch

Feel free to contact me if you have something to say!